Actin protein (biotin, rabbit skeletal muscle) AB07-C from Cytoskeleton, Inc.

Product Specs
ItemActin protein (biotin, rabbit skeletal muscle)
CompanyCytoskeleton, Inc.
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberAB07-C
Molecule NameActin
Quantity20 x 20 µg
Brand/TrademarkCytoskeleton, Inc
Source/Expression Systembiotin, rabbit skeletal muscle
SpeciesRabbit

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Cytoskeleton, Inc.

1830 S. Acoma St.

Denver, Colorado 80223

United States

Phone: 303 322-2254
303 322-2257

Company Profile

Website: http://cytoskeleton.com/

(1) High-Speed Optical Traps Address Dynamics of Processive and Non-Processive Molecular MotorsOptical TweezersFebruary 22, 2023Lucia Gardini, Michael S. Woody, Anatolii V. Kashchuk, Yale E. Goldman, E. Michael Ostap, Marco CapitanioBiotinylated F-Actin Reagents 1–6 as in Subheading 2.3.1. Biotinylated G-actin protein (Cytoskeleton, AB07), reconstituted to 10 mg/mL from powder following manufacturer instructions, final buffer: 5 mM Tris–HCl,

(2) Cell surface-bound La protein regulates the cell fusion stage of osteoclastogenesisNature communicationsFebruary 4, 2023Whitlock JM, Leikina E, Melikov K, De Castro LF, Mattijssen S, Maraia RJ, Collins MT, Chernomordik LV.aliquoted and kept at −80 °C. Recombinant, biotin tagged actin was purchased from Cytoskeleton Inc. (#AB07-A). Plasmids were introduced into primary osteoclasts at day 2 of RANKL stimulation via jetPRIME according

(3) α-catenin switches between a slip and an asymmetric catch bond with F-actin to cooperatively regulate cell junction fluidityNature CommunicationsDecember 1, 2022C. Arbore, M. Sergides, L. Gardini, G. Bianchi, A. V. Kashchuk, I. Pertici, P. Bianco, F. S. Pavone, M. Capitaniothe linker: “SDLVPRGS”). G-actin and biotinylated actin were bought from Cytoskeleton, Inc. (AKL99, AB07-A). Actin filaments were obtained by incubating 0.4 μg·μl−1 biotinylated actin and 0.2 μg·μl−1 actin in

(4) F-actin architecture determines constraints on myosin thick filament motionNat CommunNovember 16, 2022Camelia G. Muresan, Zachary Gao Sun, Vikrant Yadav, A. Pasha Tabatabai, Laura Lanier, June Hyung Kim, Taeyoon Kim, Michael P. Murrellmentioned ratio) with Biotinylated actin monomers (CYTOSKELETON INC, https://www.cytoskeleton.com/actin/ab07) at 10:1 ratio. Fluorescent beads (488 nm fluorescent green) of 0.5 μm in size that are Streptavidin-tagged

(5) Encapsulated actomyosin patterns drive cell-like membrane shape changesiScienceMay 20, 2022Yashar Bashirzadeh, Hossein Moghimianavval, Allen P. LiuAT01-A Myosin II,Cytoskeleton,Cat# MY02-A Arp2/3,Cytoskeleton,Cat# RP01P-A Actin-biotin,Cytoskeleton,Cat# AB07-A Adenosine triphosphate (ATP),Cytoskeleton,Cat# BSA04-001 Alexa Fluor 568 actin,Thermo Fisher Scientific,Cat#

(6) Secreted gelsolin inhibits DNGR-1-dependent cross-presentation and cancer immunityCellJuly 22, 2021Giampazolias E, Schulz O, Lim KHJ, Rogers NC, Chakravarty P, Srinivasan N, Gordon O, Cardoso A, Buck MD, Poirier EZ, et al.,Cytoskeleton Inc.,CF01-A Actin from skeletal muscle,Cytoskeleton Inc.,AKL99 Actin biotin-conjugated,Cytoskeleton Inc,AB07 Actin rhodamine-conjugated,Cytoskeleton Inc,AR05 Myosin II from rabbit skeletal muscle,Cytoskeleton

(7) The receptor DNGR-1 signals for phagosomal rupture to promote cross-presentation of dead-cell-associated antigensNature immunologyFebruary 1, 2021Canton J, Blees H, Henry CM, Buck MD, Schulz O, Rogers NC, Childs E, Zelenay S, Rhys H, Domart MC, et al.Inc. "Myosin II, skeletal muscle",MY02-A,Cytoskeleton Inc. "Biotinylated actin, skeletal muscle",AB07-A,Cytoskeleton Inc. Streptavidin coated beads/microspheres (2μm),24160-5,Polysciences inc. Streptavidin

(8) Molecular mechanism for direct actin force-sensing by α-catenineLifeSeptember 24, 2020Mei L, Espinosa de Los Reyes S, Reynolds MJ, Leicher R, Liu S, Alushin GM."Cytoskeleton, Inc",AR05-C, Biological sample (O. cuniculus),Actin Protein (Biotin): Rabbit Skeletal Muscle,"Cytoskeleton, Inc",AB07-C, Other,Streptavidin Coated Polystyrene Particles,Spherotech,SVP-40–5,Used in optical trapping experiments Other,C-flat

(9) Varying crosslinking motifs drive the mesoscale mechanics of actin-microtubule compositesScientific ReportsDecember 1, 2019Shea N. Ricketts, Madison L. Francis, Leila Farhadi, Michael J. Rust, Moumita Das, Jennifer L. Ross, Rae M. Robertson-Andersonskeletal actin, biotinylated actin and Alexa-488-labeled actin were purchased from Cytoskeleton (AKL99, AB07) and Thermofisher (A12373) and stored at −80 °C in a Ca buffer (2 mM Tris (pH 8.0), 0.2 mM ATP, 0.5

(10) Non-monotonic dependence of stiffness on actin crosslinking in cytoskeleton compositesSoft MatterNovember 28, 2019Madison L. Francis, Shea N. Ricketts, Leila Farhadi, Michael J. Rust, Moumita Das, Jennifer L. Ross, Rae M. Robertson-Anderson(G-actin), biotinylated G-actin and Alexa-488-labeled G-actin are purchased from Cytoskeleton (AKL99, AB07) and Thermofisher (A12373) and suspended at 1 mg/ml, 2 mg/ml and 1.5 mg/ml, respectively, in Ca Buffer

(11) Septin 2/6/7 complexes tune microtubule plus-end growth and EB1 binding in a concentration- and filament-dependent mannerMolecular Biology of the CellNovember 1, 2019Konstantinos Nakos, Megan R. Radler, Elias T. SpiliotisμM) were made by incubating 83% unlabeled G-actin (APHL99; Cytoskeleton) with 8.5% biotin-G-actin (AB07; Cytoskeleton) and 8.5% Rhodamine-G-actin (APHR; Cytoskeleton) in the presence of F-actin buffer (20

(12) Actin Dynamics, Regulated by RhoA-LIMK-Cofilin Signaling, Mediates Rod Photoreceptor Axonal Retraction After Retinal InjuryInvestigative ophthalmology & visual scienceMay 1, 2019Wang W, Halasz E, Townes-Anderson E.EGTA, 5.5mM glucose), 0.05% saponin, and freshly prepared actin dilution buffer (0.4 μM biotin-actin (AB07; Cytoskeleton, Inc.), 0.2 mM HEPES (pH 7.5), 40 μM MgCl2, 40 μM ATP). Cultures were flooded with 1×

(13) Adhesion to nanofibers drives cell membrane remodeling through one-dimensional wettingNature communicationsOctober 25, 2018Charles-Orszag A, Tsai FC, Bonazzi D, Manriquez V, Sachse M, Mallet A, Salles A, Melican K, Staneva R, Bertin A, et al.ibidi (Silicon open chambers, 80209). Biotinylated actin was purchased from Cytoskeleton (biotin-actin, AB07). Phalloidin-FluoProbes®488 was purchased from Interchim (phalloidin488, FP-JO2830). β-casein from bovine

(14) Targeting invadopodia-mediated breast cancer metastasis by using ABL kinase inhibitorsOncotargetApril 24, 2018Tomer Meirson, Alessandro Genna, Nikola Lukic, Tetiana Makhnii, Joel Alter, Ved P. Sharma, Yarong Wang, Abraham O. Samson, John S. Condeelis, Hava Gil-HennmM EGTA, 0.2 mg/ml saponin, 1 mM ATP, and 1% BSA) containing 0.4 μM biotin-conjugated muscle actin (AB07, Cytoskeleton Inc.) for 1 minute at 37° C. Cells were fixed in 3.7% paraformaldehyde for 5 minutes,

(15) Bioconjugated Core–Shell Microparticles for High‐Force Optical TrappingParticle & Particle Systems CharacterizationMarch 1, 2018Juan Carlos Cordova, Dana N. Reinemann, Daniel J. Laky, William R. Hesse, Sophie K. Tushak, Zane L. Weltman, Kelsea B. Best, Rizia Bardhan, Matthew J. Lang10 mg/mL actin (Cytoskeleton, AKL99) were mixed with 5 μL 1 mg/mL biotinylated action (Cytoskeleton, AB07). General Actin Buffer (GAB, 5 mM Tris-HCl, 0.2 mM CaCl2, 0.5 mM DTT, 0.2 mM ATP) was made, and 100

(16) Pyk2 and FAK differentially regulate invadopodia formation and function in breast cancer cellsJournal of Cell BiologyJanuary 2, 2018Alessandro Genna, Stefanie Lapetina, Nikola Lukic, Shams Twafra, Tomer Meirson, Ved P. Sharma, John S. Condeelis, Hava Gil-HennmM EGTA, 0.2 mg/ml saponin, 1 mM ATP, and 1% BSA) containing 0.4 µM biotin-conjugated muscle actin (AB07; Cytoskeleton Inc.) for 1 min at 37°C. Cells were fixed in 3.7% paraformaldehyde for 5 min, blocked

(17) Measuring the Kinetic and Mechanical Properties of Non-processive Myosins Using Optical TweezersMethods Mol. Biol.May 9, 2017Michael J. Greenberg, Henry Shuman, E. Michael Ostapestablished protocols (51). 8Biotinylated actin can be purchased from commercial sources (Cytoskeleton AB07) or prepared from purified actin (52). Methods Mol Biol. Author manuscript; available in PMC 2018 January

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Actin protein (biotin, rabbit skeletal muscle) from Cytoskeleton, Inc.

Cytoskeleton, Inc.

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Actin protein (biotin, rabbit skeletal muscle) from Cytoskeleton, Inc.

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